Coding

Part:BBa_K563010

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-04)


Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

Central role of TORC1, a highly conserved Ser/Thr protein kinase which is the central component of a major signaling transduction network that controls cell growth in diverse eukaryotic organisms, ranging from yeast to human beings. Massive physiological processes, such as amino acid biosynthesis, transcription and translation machinery, carbohydrate metabolism, nucleotide biosynthesis, stress response, protein turnover and cell cycle are directly or indirectly linked with TOR pathway.In our project, it is the target gene we want to improve, its activity is inhibited by multi-inhibitor such as acetic acid, phenol and furan.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal NheI site found at 5457
    Illegal NheI site found at 7374
    Illegal SpeI site found at 7014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal BglII site found at 2028
    Illegal BglII site found at 7296
    Illegal BamHI site found at 5643
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
    Illegal NgoMIV site found at 4996
    Illegal AgeI site found at 4236
    Illegal AgeI site found at 7393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1475
    Illegal SapI.rc site found at 4666
    Illegal SapI.rc site found at 4918


[edit]
Categories
Parameters
n/aTor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway